Linked Life Data

3081258

Source:http://linkedlifedata.com/resource/pubmed/id/3081258

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4 Pt 1
pubmed:dateCreated
1986-4-23
pubmed:abstractText
The inhibition of glycolysis in tumor cells by methionine requires that the cells be incubated with methionine for several hours in the presence of serum. We now show that in the case of confluent rat-1 fibroblasts transfected with the ras gene the serum can be substituted by insulin and insulin-like growth factor I or II. No other growth factor tested was effective. In subconfluent ras cells additional growth factors (transferrin and high density lipoproteins) were required for maximal inhibition of glycolysis by methionine. Exploration of the mechanism of action of methionine revealed that the accumulation of [35S]methionine into rat-1 fibroblasts was only marginally increased by insulin. We propose that methionine inhibits an adenosine triphosphatase activity because addition of low concentrations of Nonidet P-40 greatly enhanced glycolysis even in the presence of methionine, suggesting that it did not affect the glycolytic enzymes directly. Methionine also affected growth both in monolayer and soft agar. Rat-1 fibroblasts transfected with the ras gene were markedly more sensitive to methionine than cells transfected with the myc gene.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1800-4
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Effect of growth factors and methionine on glycolysis and methionine transport in rat fibroblasts and fibroblasts transfected with myc and ras genes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.