Linked Life Data

3077312

Source:http://linkedlifedata.com/resource/pubmed/id/3077312

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-11-27
pubmed:abstractText
In the present immunohistochemical study the occurrence and distribution of CCK-immunoreactive neurons were analyzed in the brain, spinal cord and sensory ganglia using sequence specific antisera. Thus, antibodies directed towards the C-terminal portion of CCK-33, to the N-terminal portion of CCK-8 and to the mid portion of CCK-33 as well as monoclonal antibodies were used. For comparison antisera raised against calcitonin gene-related peptide (CGRP), tyrosine hydroxylase (TH) and 5-hydroxytryptamine (5-HT) were used. Untreated, colchicine treated, 6-hydroxydopamine (6-OH-DA) treated and ibotenic acid treated rats were analyzed. The results indicate that most CCK systems in the rat central nervous system contain genuine CCK. These include, for example, the hippocampal formation, the hypothalamus, several subcortical forebrain areas, the ventral mesencephalon, nucleus tractus solitarii, some neurons in the ventral medulla oblongata as well as local and possibly descending neurons in the spinal cord. An exception was primary sensory neurons in which CCK-like immunoreactivity (LI) could only be demonstrated with C-terminally directed antisera and probably represents cross-reactivity with CGRP or a similar peptide. The central branches of such primary afferents were found both in the dorsal vagal complex, in the spinal trigeminal nucleus and in the dorsal horn of the spinal cord. Special attention was focused on CCK-LI in mesencephalic dopamine neurons and in their projection areas including nucleus accumbens, tuberculum olfactorium and particularly the caudate nucleus. In the latter structure CCK-LI exhibited a heterogenous pattern probably representing fibres of different types and origin. Thus, CCK-LI coexists with dopamine in two anatomically and morphologically distinguishable systems, one located in the periventricular area, increasing in size in the caudal direction to occupy most of the cauda, and a second system consisting of very fine dots in the medial half of the caudate nucleus. These two fibre types disappeared after 6-OH-DA treatment. A third system consisted of strongly fluorescent patches distributed at all levels of the caudate nucleus, mainly in its medial half. A diffuse, weakly fluorescent network of CCK-positive fibres was also found over the entire caudate nucleus. The latter two systems did not disappear after 6-OH-DA. Finally, local CCK-positive cell bodies were seen in small numbers, mainly in the ventral aspects of the caudate nucleus.(ABSTRACT TRUNCATED AT 400 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0891-0618
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
11-51
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
Immunohistochemical studies on cholecystokinin (CCK)-immunoreactive neurons in the rat using sequence specific antisera and with special reference to the caudate nucleus and primary sensory neurons.
pubmed:affiliation
Department of Histology, Karolinska Institute, Stockholm, Sweden.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't