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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1989-9-29
|
| pubmed:abstractText |
The correlation of the phenotypic changes of v-Ha-ras transfected cells with the expression of p21ras and the modified responses to growth factors and a tumor promoter were examined. Transfection of the v-Ha-ras gene together with the neomycin-resistance gene into 208F rat fibroblasts yielded transformed clones characterized by morphological changes, anchorage-independent growth, and tumorigenicity in nude mice. The degrees of these biological alterations were parallel with the expression of mRNA and protein of the ras gene. In ras-transformed cells, anchorage-independent growth was stimulated by epidermal growth factor (EGF), insulin, bombesin, and fibroblast growth factor, whereas in the parental 208F cells, anchorage-independent growth was observed only in the presence of EGF, and there were many fewer EGF-induced colonies than those in the ras-transformed clones. A tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA) also augmented anchorage-independent growth of ras-transformed cells and induced morphological changes in monolayer cultures without altering the expression of the ras gene or phosphorylation of the p21ras protein. Retinoic acid inhibited the TPA-induced anchorage-independent growth. These results showed a good correlation of the expression of p21ras with the phenotypic changes and the increased sensitivity of the p21ras-expressing cells to the stimulation of growth factors and tumor promoter.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bombesin,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins p21(ras),
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0899-1987
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
109-15
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3076452-Animals,
pubmed-meshheading:3076452-Blotting, Northern,
pubmed-meshheading:3076452-Bombesin,
pubmed-meshheading:3076452-Carcinogenicity Tests,
pubmed-meshheading:3076452-Carcinogens,
pubmed-meshheading:3076452-Cell Adhesion,
pubmed-meshheading:3076452-Cell Transformation, Neoplastic,
pubmed-meshheading:3076452-Cells, Cultured,
pubmed-meshheading:3076452-Epidermal Growth Factor,
pubmed-meshheading:3076452-Fibroblasts,
pubmed-meshheading:3076452-Mice,
pubmed-meshheading:3076452-Mice, Nude,
pubmed-meshheading:3076452-Oncogenes,
pubmed-meshheading:3076452-Phenotype,
pubmed-meshheading:3076452-Proto-Oncogene Proteins,
pubmed-meshheading:3076452-Proto-Oncogene Proteins p21(ras),
pubmed-meshheading:3076452-Rats,
pubmed-meshheading:3076452-Tetradecanoylphorbol Acetate,
pubmed-meshheading:3076452-Transfection
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Modified responsiveness of v-Ha-ras-transfected rat fibroblasts to growth factors and a tumor promoter.
|
| pubmed:affiliation |
Department of Cancer Cell Research, University of Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|