Linked Life Data

3072251

Source:http://linkedlifedata.com/resource/pubmed/id/3072251

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-6-5
pubmed:databankReference
pubmed:abstractText
Oligodeoxyribonucleotide-directed mutagenesis has been used to delete the leader sequence-coding region from the yeast gene specifying Mn-superoxide dismutase and thus enable its expression in Escherichia coli. The deletion of this leader was demonstrated to be an absolute requirement for the synthesis of an active yeast enzyme in the bacterium. Complementation analysis in E. coli has confirmed that the product of the truncated yeast gene is active in vivo as well as in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
121-30
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Functional expression of the yeast Mn-superoxide dismutase gene in Escherichia coli requires deletion of the signal peptide sequence.
pubmed:affiliation
Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't