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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1989-5-26
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pubmed:abstractText |
We describe the preparation of an in vitro translation system from heat shock-treated Aspergillus nidulans, capable of supporting efficient and faithful synthesis of proteins from natural and in vitro transcribed eukaryotic messages. In vitro synthesized prepro-alpha-factor was translocated across Aspergillus nidulans microsomal membranes in either the homologous A. nidulans or a yeast cell-free system. The translocated prepro-alpha-factor was protected from digestion by protease and glycosylated to higher MW forms.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0172-8083
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
561-6
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:3072100-Aspergillus nidulans,
pubmed-meshheading:3072100-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3072100-Fungal Proteins,
pubmed-meshheading:3072100-Microsomes,
pubmed-meshheading:3072100-Protein Biosynthesis,
pubmed-meshheading:3072100-Protein Precursors,
pubmed-meshheading:3072100-RNA, Fungal,
pubmed-meshheading:3072100-RNA, Messenger,
pubmed-meshheading:3072100-Saccharomyces cerevisiae Proteins
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pubmed:year |
1988
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pubmed:articleTitle |
An efficient cell-free translation system from Aspergillus nidulans and in vitro translocation of prepro-alpha-factor across Aspergillus microsomes.
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pubmed:affiliation |
Allelix Inc. Biopharmaceutical Division, Mississauga, Ontario, Canada.
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pubmed:publicationType |
Journal Article
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