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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
9
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pubmed:dateCreated |
1989-5-18
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pubmed:abstractText |
We describe the use of lectins as specific stabilizing agents for the polysaccharide capsular components of two Gram-positive bacteria, Streptococcus agalactiae and Streptococcus bovis. Treatment of bacterial suspensions with wheatgerm agglutinin and concanavalin A allowed better morphological preservation as well as immunoelectron microscopic localization of a capsular component (lipoteichoic acid) by employing specific antibodies and the protein A-gold technique. Data obtained indicate that lectins are useful agents in preserving highly water-soluble capsular components during the electron microscopy procedures for both unembedded and embedded samples.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0018-2214
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
526-30
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:3071525-Animals,
pubmed-meshheading:3071525-Concanavalin A,
pubmed-meshheading:3071525-Immunohistochemistry,
pubmed-meshheading:3071525-Lipopolysaccharides,
pubmed-meshheading:3071525-Microscopy, Electron,
pubmed-meshheading:3071525-Preservation, Biological,
pubmed-meshheading:3071525-Streptococcus agalactiae,
pubmed-meshheading:3071525-Teichoic Acids,
pubmed-meshheading:3071525-Wheat Germ Agglutinins
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pubmed:year |
1988
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pubmed:articleTitle |
Preservation of capsular material of streptococcal cells by specific lectins determined by immunoelectron microscopy.
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pubmed:affiliation |
Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy.
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pubmed:publicationType |
Journal Article
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