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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1989-3-22
|
| pubmed:abstractText |
We studied the methyl acceptor capacity of insulin and glucagon in vitro. The levels of carboxylmethylation of pancreatic hormones (dpm x 10(3], when incubated with S-adenosyl-L-(3H-methyl)-methionine as methyl donor and purified protein carboxylmethylase, were: insulin (n = 6) 8.1 +/- 0.2 and 11.1 +/- 1.5 (mean +/- SEM) for 0.25 and 1.0 mg/ml, respectively; glucagon (n = 6) 17.0 +/- 3.2 and 40.2 +/- 2.5 (mean +/- SEM) for 0.5 and 1.0 mg/ml, respectively. On a molar basis, the methyl acceptor capacity was 1.0 dpm/pmol for insulin and 9.5 dpm/pmol for glucagon. Polyacrylamide gel electrophoresis of carboxylmethylated hormones showed a radioactivity (3H-methyl) peak that co-migrated with the corresponding 125I-hormone. Glucagon, but not insulin, seems to be a relatively good substrate for carboxylmethylation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0001-5563
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
25
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
127-31
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:3066084-Adrenal Glands,
pubmed-meshheading:3066084-Animals,
pubmed-meshheading:3066084-Cattle,
pubmed-meshheading:3066084-Glucagon,
pubmed-meshheading:3066084-Insulin,
pubmed-meshheading:3066084-Methylation,
pubmed-meshheading:3066084-Protein Methyltransferases,
pubmed-meshheading:3066084-Protein O-Methyltransferase,
pubmed-meshheading:3066084-Substrate Specificity
|
| pubmed:articleTitle |
Carboxylmethylation of insulin and glucagon in vitro.
|
| pubmed:affiliation |
Departamento de Fisiología, Facultad de Medicina, Universidad de Extremadura, Badajoz, Spain.
|
| pubmed:publicationType |
Journal Article
|