Linked Life Data

3064370

Source:http://linkedlifedata.com/resource/pubmed/id/3064370

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-3-6
pubmed:abstractText
A mutation-activated human Ki-ras gene was detected in NIH 3T3 cells transfected with high molecular weight DNA extracted from peripheral blood leukocytes of a healthy blood donor. A deoxyguanosine at position 35 of the first exon was substituted by deoxythymidine. Nevertheless, cloning and sequencing of seven independent Ki-ras first exons, isolated from the same human genomic DNA used to transfect NIH 3T3 cells, failed to reveal the expected point mutation. Since transfected DNA is susceptible to mutagenesis in mammalian cells, we hypothesize that a base substitution occurred during the transfection assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0300-8916
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
499-506
pubmed:dateRevised
2008-12-12
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Activation by point mutation of Ki-ras gene occurring in transfected human normal DNA.
pubmed:affiliation
Experimental Oncology 1, Centro di Riferimento Oncologico, Aviano, Pordenone, Italia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't