| pubmed-article:3050994 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3050994 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:3050994 | lifeskim:mentions | umls-concept:C1512035 | lld:lifeskim |
| pubmed-article:3050994 | lifeskim:mentions | umls-concept:C0025462 | lld:lifeskim |
| pubmed-article:3050994 | lifeskim:mentions | umls-concept:C0000544 | lld:lifeskim |
| pubmed-article:3050994 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:3050994 | pubmed:dateCreated | 1988-11-21 | lld:pubmed |
| pubmed-article:3050994 | pubmed:abstractText | The autofluorescent serotonin analogue 5,7-dihydroxytryptamine (5,7-DHT) was used to identify living catecholaminergic neurons in monolayer cultures derived from the embryonic rat mesencephalon. A high correlation between 5,7-DHT accumulation and aldehyde-induced catecholamine fluorescence as well as tyrosine hydroxylase but not dopamine-beta-hydroxylase or phenylethanolamine-N-methyltransferase immunoreactivity was found. This indicates that these cells were dopamine-containing neurons. Whole-cell patch recordings showed that all mesencephalic neurons had resting membrane potentials of -50 mV or greater and input resistances ranging between 200 and 700 M omega and exhibited spontaneous action potentials and postsynaptic potentials. The duration of the action potential of the dopamine-containing neurons was characteristically longer than that of the non-dopamine-containing mesencephalic cells. In some dopamine-containing neurons, repolarization of the action potential was clearly biphasic, and the slow phase of repolarization was reversibly blocked by local application of Cd2+ or Co2+. This "shoulder" in the action potential was never observed in non-dopamine-containing neurons, where Cd2+ or Co2+ application was always without effect. It is concluded that 5,7-DHT can be used to identify living dopamine-containing neurons in dissociated mesencephalic cultures and these neurons express distinct electrical properties. | lld:pubmed |
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| pubmed-article:3050994 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3050994 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3050994 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3050994 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3050994 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3050994 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3050994 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:3050994 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:3050994 | pubmed:author | pubmed-author:BarkerJ LJL | lld:pubmed |
| pubmed-article:3050994 | pubmed:author | pubmed-author:MarianoA VAV | lld:pubmed |
| pubmed-article:3050994 | pubmed:author | pubmed-author:HarrisonN LNL | lld:pubmed |
| pubmed-article:3050994 | pubmed:author | pubmed-author:SilvaN LNL | lld:pubmed |
| pubmed-article:3050994 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3050994 | pubmed:volume | 85 | lld:pubmed |
| pubmed-article:3050994 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3050994 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3050994 | pubmed:pagination | 7346-50 | lld:pubmed |
| pubmed-article:3050994 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3050994 | pubmed:meshHeading | pubmed-meshheading:3050994-... | lld:pubmed |
| pubmed-article:3050994 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3050994 | pubmed:articleTitle | 5,7-Dihydroxytryptamine identifies living dopaminergic neurons in mesencephalic cultures. | lld:pubmed |
| pubmed-article:3050994 | pubmed:affiliation | Laboratory of Neurophysiology, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892. | lld:pubmed |
| pubmed-article:3050994 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3050994 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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