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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1988-11-21
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pubmed:abstractText |
The autofluorescent serotonin analogue 5,7-dihydroxytryptamine (5,7-DHT) was used to identify living catecholaminergic neurons in monolayer cultures derived from the embryonic rat mesencephalon. A high correlation between 5,7-DHT accumulation and aldehyde-induced catecholamine fluorescence as well as tyrosine hydroxylase but not dopamine-beta-hydroxylase or phenylethanolamine-N-methyltransferase immunoreactivity was found. This indicates that these cells were dopamine-containing neurons. Whole-cell patch recordings showed that all mesencephalic neurons had resting membrane potentials of -50 mV or greater and input resistances ranging between 200 and 700 M omega and exhibited spontaneous action potentials and postsynaptic potentials. The duration of the action potential of the dopamine-containing neurons was characteristically longer than that of the non-dopamine-containing mesencephalic cells. In some dopamine-containing neurons, repolarization of the action potential was clearly biphasic, and the slow phase of repolarization was reversibly blocked by local application of Cd2+ or Co2+. This "shoulder" in the action potential was never observed in non-dopamine-containing neurons, where Cd2+ or Co2+ application was always without effect. It is concluded that 5,7-DHT can be used to identify living dopamine-containing neurons in dissociated mesencephalic cultures and these neurons express distinct electrical properties.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-1123645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-13574437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-280254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-2873867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-360937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-3726538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-3796886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-3915291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-406252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-4576427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-6123092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-6676108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-7378772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3050994-804181
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7346-50
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3050994-5,7-Dihydroxytryptamine,
pubmed-meshheading:3050994-Action Potentials,
pubmed-meshheading:3050994-Animals,
pubmed-meshheading:3050994-Dihydroxytryptamines,
pubmed-meshheading:3050994-Dopamine,
pubmed-meshheading:3050994-Histocytochemistry,
pubmed-meshheading:3050994-Mesencephalon,
pubmed-meshheading:3050994-Microscopy, Fluorescence,
pubmed-meshheading:3050994-Microscopy, Phase-Contrast,
pubmed-meshheading:3050994-Neurons,
pubmed-meshheading:3050994-Rats
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pubmed:year |
1988
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pubmed:articleTitle |
5,7-Dihydroxytryptamine identifies living dopaminergic neurons in mesencephalic cultures.
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pubmed:affiliation |
Laboratory of Neurophysiology, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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