Linked Life Data

3050493

Source:http://linkedlifedata.com/resource/pubmed/id/3050493

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-11-1
pubmed:abstractText
Seven aprotic polar solvents which had previously been shown to interfere with the aggregation in vitro of porcine brain tubulin have been examined for their ability to induce mitotic aneuploidy in Saccharomyces cerevisiae in relation to temperature during exposure. Induction of aneuploidy was in general considerably enhanced when incubation at 28 degrees C was interrupted by overnight storage at low temperature (cold shock). The optimum cold-shock temperatures for individual chemicals varied over a range of 0-16 degrees C. While storage at reduced temperature enhanced the effect of treatment at 28 degrees C, it was also shown that continuous incubation at reduced temperature could greatly enhance the induction of aneuploidy. Only 2 chemicals, 1-methyl-2-pyrrolidinone and gamma-valerolactone, required cold shock to yield a positive response. The other chemicals did not require cold shock for enhanced induction. The observation that the agents examined also interfere with in vitro tubulin aggregation suggests that there is a temperature component to the interaction of these agents with tubulin in vivo. This temperature component is unusual in that the most effective temperature range for aneuploidy induction can be well below the optimal growth temperature for the test organism.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:volume
201
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
431-42
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Aprotic polar solvents that affect porcine brain tubulin aggregation in vitro induce aneuploidy in yeast cells growing at low temperatures.
pubmed:affiliation
Institute of Microbiology, Technical University of Darmstadt, F.R.G.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.