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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1978-3-21
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pubmed:abstractText |
Aortic endothelial cells from normal pigs and pigs with von Willebrand disease have been established in long-term cultures. Both cultures appeared similar in terms of general growth characteristics, morphologic features and ultrastructure. Immunofluorescent staining of these cultures with chicken (or rabbit) antiporcine ristocetin-Willebrand factor sera (or IgG) resulted in extensive perinuclear staining of the cells in both cultures. Additionally, staining of semiconfluent cultures of normal cells for ristocetin-Willebrand factor revealed an extensive meshwork of distinct, immunologically identifiable ristocetin-Willebrand factor-containing filaments between cells. Immunoreactive material was considerably decreased and more diffuse between cells in semiconfluent cultures from affected pigs. Through immunocytochemical staining with peroxidase-coupled antiserum, the filaments (of indeterminate length) were found to have a diameter of approximately 300 A. Finally, washed porcine platelets interacted extensively with scrape-damaged cultures of affected endothelial cells. This interaction of platelets with damaged normal cultures was abolished by pretreatment of the cultures with rabbit antiporcine ristocetin-Willebrand factor IgG.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-1159083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-14302464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-165826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-174237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-405441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-4107220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-4327464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-4355998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-4363161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-4542959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-4583980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-4606548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-53607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/304580-6061717
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5702-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:304580-Animals,
pubmed-meshheading:304580-Aorta,
pubmed-meshheading:304580-Blood Platelets,
pubmed-meshheading:304580-Cell Division,
pubmed-meshheading:304580-Cells, Cultured,
pubmed-meshheading:304580-Endothelium,
pubmed-meshheading:304580-Microscopy, Electron,
pubmed-meshheading:304580-Ristocetin,
pubmed-meshheading:304580-Swine,
pubmed-meshheading:304580-von Willebrand Diseases,
pubmed-meshheading:304580-von Willebrand Factor
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pubmed:year |
1977
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pubmed:articleTitle |
Cultured aortic endothelial cells from pigs with von Willebrand disease: in vitro model for studying the molecular defect(s) of the disease.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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