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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1988-10-13
|
| pubmed:abstractText |
Tumor cell variants which grow adherent to a plastic surface could be isolated in a reproducible way from the high metastatic tumor cell line ESb which grows in a suspension culture. This occurred when starting selection from the uncloned parental line as well as from a freshly derived non-adhesive subclone. The variants showed changes in their karyotype. These were quantitative (tetraploidization) and qualitative (single chromosome aberrations involving the chromosomes 12 and 17 and a marker MX-7). Phenotypic cell surface changes were documented in vitro by immunofluorescence using a monoclonal antibody (mAb 12-15) directed against a distinct plasma membrane glycoprotein of 60-69kD (gp 60-69). The expression of gp 60-69 increased with time of selection for adherence to plastic surface. The adherent cells showed in all cases a greatly reduced overall malignancy as seen by a prolonged survival time of respective tumor bearing animals compared with the suspension growing parental cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0262-0898
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
485-99
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3044658-Animals,
pubmed-meshheading:3044658-Antibodies, Monoclonal,
pubmed-meshheading:3044658-Cell Adhesion,
pubmed-meshheading:3044658-Cell Line,
pubmed-meshheading:3044658-Chromosomes,
pubmed-meshheading:3044658-Clone Cells,
pubmed-meshheading:3044658-Fluorescent Antibody Technique,
pubmed-meshheading:3044658-Karyotyping,
pubmed-meshheading:3044658-Membrane Glycoproteins,
pubmed-meshheading:3044658-Mice,
pubmed-meshheading:3044658-Mice, Inbred DBA,
pubmed-meshheading:3044658-Neoplasm Metastasis,
pubmed-meshheading:3044658-Neoplasm Proteins,
pubmed-meshheading:3044658-Neoplasm Transplantation,
pubmed-meshheading:3044658-Neoplasms, Experimental,
pubmed-meshheading:3044658-Phenotype,
pubmed-meshheading:3044658-Rats,
pubmed-meshheading:3044658-Rats, Inbred Strains,
pubmed-meshheading:3044658-Tumor Cells, Cultured
|
| pubmed:articleTitle |
Generation of adhesive tumor variants: chromosomal changes, reduction in malignancy and increased expression of a distinct membrane glycoprotein.
|
| pubmed:affiliation |
Institut für Immunologie und Genetik, Deutsches Krebsforschungszentrum, Heidelberg, F.R. Germany.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|