Linked Life Data

3038937

Source:http://linkedlifedata.com/resource/pubmed/id/3038937

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1987-8-28
pubmed:abstractText
We applied an indirect immunoperoxidase staining method for the detection of BK virus (BKV) antigens in Vero cells. By immunoperoxidase staining of sequential samples, expression of BKV T- and structural antigens was first detectable 72 h postinfection (pi). The initial low number of antigen positive cells was slowly increasing until 6 d pi. Thereafter, a steady progression was observed until total cytopathogenic effect 30 d pi. Viral hemagglutinins were detected 11 d pi in both the culture medium and in the cell lysate. Passage of the preparations into fresh Vero cells and immunoperoxidase detection demonstrated the presence of infectious virions in both the cell lysates and the medium from 2 d pi. The number of infectious particles in both media and cell lysates correlated to the number of IP stained cells in the sequential staining. We suggest that this type of assay might be used to examine the effects, in vitro, of interferons and other virostatic drugs of BKV and other viruses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-46
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
BK virus in cell culture: infectivity quantitation and sequential expression of antigens detected by immunoperoxidase staining.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't