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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1987-9-24
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pubmed:abstractText |
Derivatives of phage M13 were constructed and used for the in vitro preparation of heteroduplex DNA molecules containing base/base mismatches that mimick DNA lesions caused by hydrolytic deamination of 5-meC residues in Escherichia coli DNA (i.e. they carry a T/G mismatch in the special sequence context provided by the recognition site -CCA/TGG-of the Dcm-methyltransferase). Upon introduction of these heteroduplex DNAs into CaCl2-treated E. coli cells, the mismatches are efficiently repaired with high bias in favour of the DNA strand containing the mismatched guanine residue. This special DNA mismatch-repair operates on fully dam-methylated DNA and is independent of gene mutH. It thus fulfills the salient requirements of a repair pathway responsible for counteracting the spontaneous hydrolytic deamination of 5-meC in vivo. The repair efficiency is boosted by a 5-methyl group present on the cytosine residue at the next-nearest position to the 5' side of the mismatched guanine. The repair is severely impaired in host strains carrying a mutation in any of the three loci dcm, mutL and mutS.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-1069303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-1093175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-16593539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-2951250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-2993796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-3014530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-3523187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-355893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-6096830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-6225003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-6225697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-6295880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-6313347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-6386179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3038536-6999365
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1809-15
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:3038536-5-Methylcytosine,
pubmed-meshheading:3038536-Amino Acid Sequence,
pubmed-meshheading:3038536-Bacterial Proteins,
pubmed-meshheading:3038536-Base Sequence,
pubmed-meshheading:3038536-Coliphages,
pubmed-meshheading:3038536-Cytosine,
pubmed-meshheading:3038536-DNA Repair,
pubmed-meshheading:3038536-Escherichia coli,
pubmed-meshheading:3038536-Genes,
pubmed-meshheading:3038536-Genes, Bacterial,
pubmed-meshheading:3038536-Hydrolysis,
pubmed-meshheading:3038536-Mutation
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pubmed:year |
1987
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pubmed:articleTitle |
DNA mismatch-repair in Escherichia coli counteracting the hydrolytic deamination of 5-methyl-cytosine residues.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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