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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1987-8-26
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA clone for a type II regulatory (R) subunit of the cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) was isolated from a rat skeletal muscle library using a specific 47-base oligonucleotide probe. The rat cDNA was 1.2 kilobases (kb) in length and contained an open reading frame of 1.113 kb representing 92% of the coding region of the molecule. Nick-translated rat cDNA was then used to isolate a mouse RII cDNA clone from a brain library that contained an open reading frame of 1.143 kb. Because both cDNAs lacked complete coding sequences, the remainder of the RII coding region was obtained from a 15-kb mouse genomic clone. The mouse RII coding region contains 1.2 kb corresponding to a 400-amino acid protein of 51.141 kDa. The mouse cDNA hybridizes to two mRNA species, a 2.4-kb form that was only observed in testis and a 6.0-kb form found in a wide range of tissues, including testis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-166986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-206557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-220219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-2416464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-2427518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-2431926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-2987223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-3023318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-3023347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-3456589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-38740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-3934166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-4030778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-6088506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-6185958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-6190178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-6245082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-6386045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-6487597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-6548414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-6577433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-7063411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3037538-7410357
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5192-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:3037538-Amino Acid Sequence,
pubmed-meshheading:3037538-Animals,
pubmed-meshheading:3037538-Base Sequence,
pubmed-meshheading:3037538-Brain,
pubmed-meshheading:3037538-Cattle,
pubmed-meshheading:3037538-Cloning, Molecular,
pubmed-meshheading:3037538-DNA Restriction Enzymes,
pubmed-meshheading:3037538-Macromolecular Substances,
pubmed-meshheading:3037538-Mice,
pubmed-meshheading:3037538-Muscles,
pubmed-meshheading:3037538-Protein Kinases,
pubmed-meshheading:3037538-Rats,
pubmed-meshheading:3037538-Swine
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pubmed:year |
1987
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pubmed:articleTitle |
The molecular cloning of a type II regulatory subunit of the cAMP-dependent protein kinase from rat skeletal muscle and mouse brain.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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