Linked Life Data

3036667

Source:http://linkedlifedata.com/resource/pubmed/id/3036667

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-8-5
pubmed:databankReference
pubmed:abstractText
The structure of the amdS gene of Aspergillus nidulans has been determined by nucleotide sequence analysis. The coding sequence is interrupted by three small introns with splicing signals consistent with other fungal genes. Possible TATA and CAAT elements are found upstream of the start point of transcription. Sequence changes in mutations in the 5' region of the gene have been determined. Two deletions including the start point of transcription abolish detectable transcripts. A series of mutations in the first exon cause frame shifts leading to polypeptide chain termination in the N-terminal region of the polypeptide. Levels of amdS RNA are reduced in these mutants indicating that translational termination can lead to decreased transcription or (more probably) to lower mRNA stability. The amdI18 mutation has properties consistent with it having a general up-promoter effect on amdS expression and results from a single base pair substitution approx. 77 bp upstream from the transcription start point.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-71
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
The nucleotide sequence of the amdS gene of Aspergillus nidulans and the molecular characterization of 5' mutations.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't