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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1987-8-10
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pubmed:abstractText |
Expression plasmids containing human interleukin-2(IL-2) cDNA under the control of viral promoters (SV40 early region, MuLV LTR, HTLV-I LTR, and ASV (Y73) LTR) were introduced into TK- mouse L cells and human FL cells to establish IL-2 producing cells. The highest levels of IL-2 producing clones were obtained in TK+ mouse L cells transformed with a recombinant plasmid having MuLV LTR as a promoter, whereas transformed cells of human FL cells (G418r) were revealed to produce IL-2 at the highest level when the cells were transfected with a plasmid containing HTLV LTR as a promoter. These results suggest that these promoter/enhancer regions possess different cell specificities in gene expression. To obtain higher levels of IL-2 production using gene amplification, the hybrid plasmids containing the hamster DHFR and human IL-2 genes were constructed and transfected into DHFR- CHO cells. DHFR+ colonies produced IL-2 at about the same level as that produced by TK+ L cells transformed with the recombinants containing MuLV LTR. Selection of methotrexate-resistant cells resulted in a 5- to 30-fold increase of IL-2 production. These cells produced IL-2 stably for at least 3 months, even in the absence of methotrexate.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0386-7196
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
205-17
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:3036377-Animals,
pubmed-meshheading:3036377-Cloning, Molecular,
pubmed-meshheading:3036377-DNA,
pubmed-meshheading:3036377-Gene Amplification,
pubmed-meshheading:3036377-Genetic Engineering,
pubmed-meshheading:3036377-Genetic Vectors,
pubmed-meshheading:3036377-Humans,
pubmed-meshheading:3036377-Interleukin-2,
pubmed-meshheading:3036377-L Cells (Cell Line),
pubmed-meshheading:3036377-Mice,
pubmed-meshheading:3036377-Plasmids,
pubmed-meshheading:3036377-Promoter Regions, Genetic,
pubmed-meshheading:3036377-Simian virus 40,
pubmed-meshheading:3036377-Thymidine Kinase
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pubmed:year |
1987
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pubmed:articleTitle |
The establishment of IL-2 producing cells by genetic engineering.
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pubmed:publicationType |
Journal Article
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