Linked Life Data

3035716

Source:http://linkedlifedata.com/resource/pubmed/id/3035716

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4806
pubmed:dateCreated
1987-7-6
pubmed:abstractText
The molecular analysis of genes identified by mutations is a major problem in mammalian genetics. As a step toward this goal, preparative field inversion gel electrophoresis (FIGE) was used to selectively isolate clones from the environment of genetically linked markers, and to select a subset of these clones containing sequences next to specific restriction sites rare in mammalian DNA. This approach has been used to generate a library highly enriched in sequences closely linked to the cystic fibrosis marker met. One clone derived from the end of a Not I restriction fragment containing the met sequence was analyzed in detail and localized within a long range map to a position 300 kilobase pairs 5' of the metD sequence.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0036-8075
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
236
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1305-8
pubmed:dateRevised
2007-3-19
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Derivation of clones close to met by preparative field inversion gel electrophoresis.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't