Linked Life Data

303569

Source:http://linkedlifedata.com/resource/pubmed/id/303569

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1978-1-27
pubmed:abstractText
Human Ia-like alloantigens have been solubilized from membranes of B lymphoblastoid cell lines using sodium deoxycholate (DOC). They have been purified by affinity chromatography using specific rabbit antibodies bound to an agarose column, eluting the antigens at pH 11.0 in the presence of 0.5% DOC. The isolated, purified, material contained two proteins of molecular weights 35 000 and 27 000 by sodium dodecyl sulfate gel electrophoresis, apparently noncovalently associated with each other. The molecules were completely separated from the soluble products of the HLA-A, B and C loci and retained serological activity as measured by their capacity to inhibit the lysis of B lymphoblastoid cell lines by B cell-specific alloantisera.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
636-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Human B cells alloantigens; separation from other membrane molecules by affinity chromatography.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.