Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-5-26
pubmed:abstractText
The cotransfection of selectable marker genes and the gene for the nonstructural proteins NS1 and NS2 of the autonomous parvovirus H-1 failed to produce cell lines that constitutively expressed NS1. A plasmid, pP38NS1cat, was constructed that expressed the NS1-NS2 gene from the H-1 P38 coat protein promoter in place of the natural P4 promoter. The P38 promoter is constitutively weak and is trans-activated by NS1. Stable cell lines were isolated that contained pP38NS1cat that was constitutively silent, but inducible with exogenous NS1 by superinfection or by treatment with sodium butyrate. The cells that were induced for this self-stimulatory genetic circuit did not remain in the culture, suggesting that expression of NS1-NS2 is cytotoxic or that the expression is not sustained. The properties of these cell lines and an example of the construction of a cell line inducible for expression of the viral coat protein gene and the bacterial gene for chloramphenicol acetyltransferase (cat) are described.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-1255851, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-13911591, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-2939261, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-2942705, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-3009844, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-3018288, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-3491293, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-3502703, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-3855242, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-3859838, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-3951993, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-4020958, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-4020972, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-4062947, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-6090130, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-6092662, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-6286831, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-6298737, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-6316266, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-6319019, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-6481856, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-6481857, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-6623929, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-667927, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-667928, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-6823009, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-7017722, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-7269249, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-7332929, http://linkedlifedata.com/resource/pubmed/commentcorrection/3033274-886304
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1448-56
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Construction of a genetic switch for inducible trans-activation of gene expression in eucaryotic cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.