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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1987-5-8
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pubmed:abstractText |
We have recently obtained 18 distinct cDNA clones representing different genes expressed in the early phase of EBV infection. One of them, c37, which is situated at the position 12907-122451 in the B95-8 viral genome, is shown here to code for a viral desoxyribonuclease [DNase]. Cell free translation of c37-selected messenger RNA yielded a protein of about 52 KDa which was immunoprecipitated by a high EA titer serum from nasopharyngeal carcinoma patient. This protein showed a DNase activity which was resistant to high salt concentrations (150 to 300 mM KCl) and was specifically neutralized by EA positive serum. These properties are typical of the EBV-specific DNase activity that we recently described in chemically induced EBV-transformed lymphoid cells. The same results were obtained on cell-free translation of the native RNA synthesized in vitro from pGEM-37 plasmid containing the entire c37 cDNA sequence (1.53 Kb). These data indicate that the BGLF5 open reading frame contained in c37 encodes for the EBV-specific DNase.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-204874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-227441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-2985823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-2990591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-3010237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-4160230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6087149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6094953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6188862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6243430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6247825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6249024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6251548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6256952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6264134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6278703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6288806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6291060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6298456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6319766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031609-6396507
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2707-17
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3031609-Base Sequence,
pubmed-meshheading:3031609-Cell Line,
pubmed-meshheading:3031609-Cell Transformation, Viral,
pubmed-meshheading:3031609-Cloning, Molecular,
pubmed-meshheading:3031609-DNA,
pubmed-meshheading:3031609-Deoxyribonucleases,
pubmed-meshheading:3031609-Genes,
pubmed-meshheading:3031609-Genes, Viral,
pubmed-meshheading:3031609-Herpesvirus 4, Human,
pubmed-meshheading:3031609-Nucleic Acid Hybridization,
pubmed-meshheading:3031609-Protein Biosynthesis,
pubmed-meshheading:3031609-Transcription, Genetic
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pubmed:year |
1987
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pubmed:articleTitle |
Identification of an Epstein-Barr virus-specific desoxyribonuclease gene using complementary DNA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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