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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1987-4-27
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pubmed:databankReference |
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pubmed:abstractText |
Sucl+ was originally identified as a DNA sequence that, at high copy number, rescued Schizosaccharomyces pombe strains carrying certain temperature-sensitive alleles of the cdc2 cell cycle control gene. We determined the nucleotide sequence of a 1,083-base-pair Sucl+ DNA fragment and S1 mapped its 866-nucleotide RNA transcript. The protein-coding sequence of the gene is interrupted by two intervening sequences of 115 and 51 base pairs. The predicted translational product of the gene is a protein of 13 kilodaltons. A chromosomal gene disruption of Sucl+ was constructed in a diploid S. pombe strain. Germinating spores carrying a null allele of the gene were capable of very limited cell division, following which many cells became highly elongated. The Sucl+ gene was also strongly overexpressed under the control of a heterologous S. pombe promoter. Overexpression of Sucl+ is not lethal but causes a division delay such that cells are approximately twice the normal length at division. These data suggest that Sucl+ encodes a protein which plays a direct role in the cell division cycle of S. pombe.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-16453669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-283395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-3010051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-3516412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-357984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-3796591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-3870979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-3889921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-4006903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6094012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6096224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6260582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6260957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6294466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6310324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6323249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6327053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6337726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6361575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6365330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6526270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6757758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-6791577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-7254352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-7262540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031478-922889
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
504-11
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:3031478-Alleles,
pubmed-meshheading:3031478-Amino Acid Sequence,
pubmed-meshheading:3031478-Base Sequence,
pubmed-meshheading:3031478-Cell Cycle,
pubmed-meshheading:3031478-Cell Cycle Proteins,
pubmed-meshheading:3031478-DNA Restriction Enzymes,
pubmed-meshheading:3031478-Fungal Proteins,
pubmed-meshheading:3031478-Genes,
pubmed-meshheading:3031478-Genes, Fungal,
pubmed-meshheading:3031478-Molecular Weight,
pubmed-meshheading:3031478-Nucleic Acid Hybridization,
pubmed-meshheading:3031478-Saccharomycetales,
pubmed-meshheading:3031478-Schizosaccharomyces,
pubmed-meshheading:3031478-Schizosaccharomyces pombe Proteins
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pubmed:year |
1987
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pubmed:articleTitle |
Sucl+ encodes a predicted 13-kilodalton protein that is essential for cell viability and is directly involved in the division cycle of Schizosaccharomyces pombe.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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