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rdf:type |
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lifeskim:mentions |
umls-concept:C0035711,
umls-concept:C0036025,
umls-concept:C0079429,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C0521449,
umls-concept:C0521451,
umls-concept:C1514873,
umls-concept:C1537998,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1880022
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pubmed:issue |
1
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pubmed:dateCreated |
1987-4-27
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pubmed:abstractText |
The mod5-1 mutation is a nuclear mutation in Saccharomyces cerevisiae that reduces the biosynthesis of N6-(delta 2-isopentenyl)adenosine in both cytoplasmic and mitochondrial tRNAs to less than 1.5% of wild-type levels. The tRNA modification enzyme, delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase, cannot be detected in vitro with extracts from mod5-1 cells. A characterization of the MOD5 gene would help to determine how the same enzyme activity in different cellular compartments can be abolished by a single nuclear mutation. To that end we have cloned the MOD5 gene and shown that it restores delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase activity and N6-(delta 2-isopentenyl)adenosine to tRNA in both the mitochondria and the nucleus/cytoplasm compartments of mod5-1 yeast cells. That MOD5 sequences are expressed in Escherichia coli and can complement an N6-(delta 2-isopentenyl)-2-methylthioadenosine-deficient E. coli mutant leads us to conclude that MOD5 is the structural gene for delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-1055375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-10793751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-223883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-2420798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-242273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-2426253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-2937632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-3031457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-337115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-347451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-3521891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-358198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-364426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-3881278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-4196579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-4292002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-4297772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-5572614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6185959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6244896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6246361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6262734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6269464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6273866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6287231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6294669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6297790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6336730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-6397370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-7028736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-7039847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-7050116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-7074684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-7363329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-781620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-845942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3031456-93644
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-84
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3031456-Alkyl and Aryl Transferases,
pubmed-meshheading:3031456-Cytoplasm,
pubmed-meshheading:3031456-DNA Restriction Enzymes,
pubmed-meshheading:3031456-Genes,
pubmed-meshheading:3031456-Genes, Fungal,
pubmed-meshheading:3031456-Genetic Complementation Test,
pubmed-meshheading:3031456-Mitochondria,
pubmed-meshheading:3031456-RNA, Transfer,
pubmed-meshheading:3031456-Saccharomyces cerevisiae,
pubmed-meshheading:3031456-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:3031456-Transferases
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pubmed:year |
1987
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pubmed:articleTitle |
Isolation and characterization of MOD5, a gene required for isopentenylation of cytoplasmic and mitochondrial tRNAs of Saccharomyces cerevisiae.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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