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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1987-5-21
|
| pubmed:abstractText |
The influence of serum from patients with essential hypertension on [3H]ouabain binding to isolated (Na+ +K+)-ATPase and on the reactivity with digoxin-specific antibodies was investigated. [3H]Ouabain binding to (Na+ +K+)-ATPase was significantly decreased (P less than 0.001) by sera of 18 hypertensive patients (34.9 +/- 1.5 pmol/U) as compared with 22 normotensive controls (43.8 +/- 1.2 pmol/U). The factor, whose concentration is increased in the serum of patients with essential hypertension, competed with [3H]ouabain at isolated (Na+ +K+)-ATPase. Therefore, it was possible to quantify this "digitalis-like" factor with a receptor assay in ouabain equivalents. Three times higher mean serum levels were found in hypertensive patients (234.8 +/- 48.7 nM) than in normotensive controls (76.3 +/- 9.3 nM). Deproteinization of the sera by ultrafiltration through steroid-adsorbing membranes and by boiling of acidified sera for 10 min led to a significant reduction of the inhibitory activity and to the complete loss of a difference between the sera of normotensives and hypertensives. After deproteinization by boiling for 15 min, sera of normotensives showed levels of "digitalis-like" compounds of 16.53 +/- 2.15 nM and hypertensives of 41.65 +/- 8.41 nM (P less than 0.05). Though significantly elevated concentrations of "digitalis-like" factor were measured with the receptor assay, no significant increase of digoxin-like activity could be detected with digoxin-specific antibodies in untreated serum.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cardenolides,
http://linkedlifedata.com/resource/pubmed/chemical/Digoxin,
http://linkedlifedata.com/resource/pubmed/chemical/Ouabain,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Drug,
http://linkedlifedata.com/resource/pubmed/chemical/Saponins,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Exchanging ATPase,
http://linkedlifedata.com/resource/pubmed/chemical/digitalis receptor,
http://linkedlifedata.com/resource/pubmed/chemical/digoxin-like factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0023-2173
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
16
|
| pubmed:volume |
65
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
179-84
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3031360-Adolescent,
pubmed-meshheading:3031360-Adult,
pubmed-meshheading:3031360-Binding, Competitive,
pubmed-meshheading:3031360-Blood Pressure,
pubmed-meshheading:3031360-Blood Proteins,
pubmed-meshheading:3031360-Cardenolides,
pubmed-meshheading:3031360-Digoxin,
pubmed-meshheading:3031360-Female,
pubmed-meshheading:3031360-Humans,
pubmed-meshheading:3031360-Hypertension,
pubmed-meshheading:3031360-Kinetics,
pubmed-meshheading:3031360-Male,
pubmed-meshheading:3031360-Middle Aged,
pubmed-meshheading:3031360-Ouabain,
pubmed-meshheading:3031360-Radioligand Assay,
pubmed-meshheading:3031360-Receptors, Drug,
pubmed-meshheading:3031360-Saponins,
pubmed-meshheading:3031360-Sodium-Potassium-Exchanging ATPase
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
A quantitative receptor assay for "digitalis-like" compounds in serum. Demonstration of raised concentrations in essential hypertension and correlation with arterial blood pressure.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|