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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1987-3-13
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pubmed:abstractText |
Mutants of Methanococcus voltae were isolated that were resistant to the coenzyme M (CoM; 2-mercaptoethanesulfonic acid) analog 2-bromoethanesulfonic acid (BES). The mutants displayed a reduced ability to accumulate [35S]BES relative to the sensitive parental strain. BES inhibited methane production from CH3-S-CoM in cell extracts prepared from wild-type sensitive or resistant strains. BES uptake required the presence of both CO2 and H2 and was inhibited by N-ethylmaleimide and several reagents that are known to disrupt energy metabolism. The mutants showed normal uptake of isoleucine and were not cross-resistant to either azaserine or 5-methyltryptophan and, thus, were neither defective in general energy-dependent substrate transport nor envelope permeability. Both HS-CoM and CH3-S-CoM prevented the uptake of BES and protected cells from inhibition by it. We propose that M. voltae has an energy-dependent, carrier-mediated uptake system for HS-CoM and CH3-S-CoM which can also mediate uptake of BES.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-104960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-16346342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-16346835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-16347059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-33148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-3764632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-390357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-3996416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-4037983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-4368921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-6236847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-6313605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-6352676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-6403944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-6801012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-6841312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-827241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-942051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3027043-98178
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
660-5
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:3027043-Alkanesulfonates,
pubmed-meshheading:3027043-Alkanesulfonic Acids,
pubmed-meshheading:3027043-Amino Acids,
pubmed-meshheading:3027043-Biological Transport,
pubmed-meshheading:3027043-Drug Resistance, Microbial,
pubmed-meshheading:3027043-Ethylmaleimide,
pubmed-meshheading:3027043-Euryarchaeota,
pubmed-meshheading:3027043-Mercaptoethanol,
pubmed-meshheading:3027043-Mesna,
pubmed-meshheading:3027043-Mutation,
pubmed-meshheading:3027043-Oxidoreductases,
pubmed-meshheading:3027043-Species Specificity
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pubmed:year |
1987
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pubmed:articleTitle |
Characterization of bromoethanesulfonate-resistant mutants of Methanococcus voltae: evidence of a coenzyme M transport system.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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