Linked Life Data

3026610

Source:http://linkedlifedata.com/resource/pubmed/id/3026610

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-3-18
pubmed:abstractText
Seven clonally derived human amniocyte cell lines from independent Simian virus-40 transformation events were analyzed for chromosomal aberrations in the precrisis stage. The distribution of chromosomal aberrations over the haploid complement was nonrandom. With increasing passage number the number of involved chromosomes increased, but the type of aberrations were the same: dicentric chromosomes and translocations due, respectively, to fusion of telomeres and breakage in the centromere regions. Interruptions to telomeres were also evident from extrachromosomal material terminally on the p arms, especially, of chromosomes #7 and #11. The cytogenetic similarities between the present results and those in transfection experiments are mentioned. All seven clones became highly heterogeneous over 10 to 20 passages (i.e., 30-60 population doublings) when, in general, transformed amniocytes enter crisis. The chromosomes which were nonrandomly involved in aberrations exhibited great diversity in types of rearrangements. Thus, for each clone multiple different rearrangements for specific chromosomes were observed in different cells. Most of these aberrations had a single step origin, which implies that breakage occurred repeatedly for the same chromosome in different cells, suggesting that the specific chromosome had become unstable in an earlier cell generation. Thus, we observed development of clonal chromosome instability, a phenomenon not reported for viral transformed cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0165-4608
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-59
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Chromosome instability in cell lineages of amniocyte clones morphologically transformed by Simian virus 40.
pubmed:publicationType
Journal Article