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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1987-1-28
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pubmed:abstractText |
A phosphatidylinositol-4-phosphate (PIP) kinase activity was purified from rat brain extract through several chromatographic steps to yield an active preparation (specific activity 1 mumol of 32P incorporated into phosphatidylinositol 4,5-bisphosphate/min per mg of protein) with an apparent molecular size of 100-110 kDa in the native form. The isolated PIP kinase required Mg2+ (optimally 20-30 mM) for its activity and was not influenced by Ca2+. The enzyme used ATP (Km 25 microM) and GTP (Km 133 microM) as phosphate sources and appeared specific for PIP (Km 3.3 micrograms/ml) as the lipid substrate. The PIP-phosphorylation reaction was inhibited by micromolar concentrations of heparin [ID50 (concn. giving 50% inhibition) 2 micrograms/ml] and the flavonoid quercetin (ID50 0.2 microM). Whereas heparin behaves as a competitive inhibitor to PIP, quercetin was competitive towards ATP (or GTP). Phosphorylation of the preparation by a highly active purified protein kinase C did not detectably alter PIP kinase activity. Whereas 12-O-tetradecanoylphorbol acetate and various phospholipids had no effect, phosphatidylserine elicited a dose-dependent activation of PIP activity. This suggests that a phosphatidylserine-PIP kinase interaction may be considered as a possible regulatory process at the cell-membrane level.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-12747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-190354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-192316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-2987958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-2988532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-2991221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-4204102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-4295336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6091634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6093768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6093804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6095092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6146314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6161840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6190690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6198580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6232463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6240267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6250080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6284174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6297477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6297738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6308167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6316323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6323423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6326105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6326140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6331677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-6828143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3026313-7142151
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
237
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3026313-Animals,
pubmed-meshheading:3026313-Brain,
pubmed-meshheading:3026313-Heparin,
pubmed-meshheading:3026313-Kinetics,
pubmed-meshheading:3026313-Magnesium,
pubmed-meshheading:3026313-Nucleotides,
pubmed-meshheading:3026313-Phospholipids,
pubmed-meshheading:3026313-Phosphotransferases,
pubmed-meshheading:3026313-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:3026313-Quercetin,
pubmed-meshheading:3026313-Rats,
pubmed-meshheading:3026313-Spermine,
pubmed-meshheading:3026313-Substrate Specificity
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pubmed:year |
1986
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pubmed:articleTitle |
Catalytic properties of a purified phosphatidylinositol-4-phosphate kinase from rat brain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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