Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1977-10-14
pubmed:abstractText
A photoreactive derivative of epidermal growth factor (EGF) has been used to identify and specifically label a membrane receptor for EGF on mouse 3T3 cells. Photoactivable EGF, labeled with 125I, was incubated with 3T3 cells and then photolyzed in situ to generate a nitrene capable of reacting with a wide variety of chemical bonds. Analysis of the system by sodium dodecyl sulfate/polyacrylamide gel electrophoresis revealed, besides the band of EGF, only one other major radioactive band, at a position indicating an apparent molecular weight of 190,000. This band was absent when a nonresponsive and nonbinding variant of 3T3 was used. A direct proportionality between binding activity and crosslinked complex formation was demonstrated using a variety of binding conditions. "Down regulated" cells, in which EGF binding activity was greatly reduced by prolonged incubation with an appropriate concentration of EGF, also had a decrease in covalent complex formation proportional to the decrease in EGF binding activity.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-1055407, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-1069998, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-1126952, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-1202035, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-1267788, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-13880319, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-14143225, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-14155, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-15625883, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-165185, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-187944, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4117794, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4296516, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4328443, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4342239, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4354860, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4376663, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4449482, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4504339, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4507606, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4517671, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4636326, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4636327, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4646881, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4745660, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-4857518, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-5039956, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-5066542, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-944704, http://linkedlifedata.com/resource/pubmed/commentcorrection/302450-977646
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2790-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Specific radiolabeling of a cell surface receptor for epidermal growth factor.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.