Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1987-1-14
pubmed:abstractText
This study demonstrates that blastocyst-derived embryonic stem cells (ES cells) can be used as a vehicle for transgenesis. The method is nearly as efficient as other methods, and the introduced neomycin phosphotransferase (neo) gene is stably transmitted through several generations with no apparent loss in G418 resistance. An important factor contributing to the efficiency of this process is the rigorous selection, before blastocyst injection, of genetically transformed cells for in vitro developmental pluripotency. One of the advantages of the ES cell route to transgenesis is that it provides investigators with the opportunity to screen for the desired genetic alterations before reintroducing the ES cells into the animal.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-1055416, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-205875, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-210957, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-2419128, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-2420518, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-2985274, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-2995814, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-3455774, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-3862122, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-3868502, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-3897439, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6095270, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6261253, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6272287, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6276022, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6577257, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6589587, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6678608, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6714319, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6796959, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6945481, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6946453, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-6950406, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-7197324, http://linkedlifedata.com/resource/pubmed/commentcorrection/3024164-7242681
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9065-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Transgenesis by means of blastocyst-derived embryonic stem cell lines.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't