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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1987-1-20
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pubmed:databankReference |
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pubmed:abstractText |
In the DNAs of all Ph1-positive chronic myelocytic leukemia patients studied to date, a breakpoint on chromosome 22 (the Ph1 chromosome) can be demonstrated with a probe from the bcr (breakpoint cluster region). Although the K562 cell line was established from cells of a chronic myelocytic leukemia patient, we have been unable to detect the Ph1 chromosome by cytogenetic means. Employing a probe from the 5' region of bcr, we have cloned an amplified Ph1 breakpoint fragment from K562. This demonstrates that K562 contains multiple remnants of a Ph1 chromosome with a breakpoint within bcr and thus may serve as a model system for the study of Ph1-positive chronic myelocytic leukemia at a molecular level. The isolation of bcr cDNA sequences shows that parts of bcr encode a protein. Employing K562, we demonstrate the presence of an abnormally sized mRNA species hybridizing to c-abl and to a bcr cDNA probe, indicating the possible consequence of the Ph1 translocation on a transcriptional level in chronic myelocytic leukemia. The isolation and sequencing of a cDNA containing the breakpoint area of this mRNA provide further evidence for its chimeric structure. Cloning of large stretches of chromosomal DNA flanking bcr and c-abl sequences in K562 and identification of the exons participating in the formation of the chimeric mRNA shows that a splice of at least 99 kilobases is made to fuse the 3' bcr exon to the 5' c-abl exon. Furthermore two chimeric cDNAs were isolated containing chromosome 9 sequences that map 43.5 kilobases downstream from the K562 breakpoint. These chromosome 9 sequences neither hybridize to the 8.5-kilobase chimeric c-abl mRNA nor to normal c-abl mRNAs in Hela cells and probably represent incorrect splicing products present in the K562 cell line.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-163658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-2989692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-2989703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-3864009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-4039028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-4126434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6091052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6091099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6142307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6159595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6193890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6198242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6204766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6260957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6266915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6281890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6299580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6300662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6302194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6306134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6308652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6316147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6319012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6319018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6580644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6587568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6772444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6791577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-699045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-6996765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-7121606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023859-912752
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
607-16
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3023859-Amino Acid Sequence,
pubmed-meshheading:3023859-Base Sequence,
pubmed-meshheading:3023859-Cell Line,
pubmed-meshheading:3023859-Chimera,
pubmed-meshheading:3023859-Chromosome Aberrations,
pubmed-meshheading:3023859-Chromosomes, Human, Pair 22,
pubmed-meshheading:3023859-Cloning, Molecular,
pubmed-meshheading:3023859-DNA,
pubmed-meshheading:3023859-DNA Restriction Enzymes,
pubmed-meshheading:3023859-Humans,
pubmed-meshheading:3023859-Leukemia, Myeloid,
pubmed-meshheading:3023859-Nucleic Acid Hybridization,
pubmed-meshheading:3023859-Transcription, Genetic
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pubmed:year |
1986
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pubmed:articleTitle |
The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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