|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1987-1-16
|
|
pubmed:abstractText |
A derivative of the myeloproliferative sarcoma virus (Neor-MPSV) carrying the mos oncogene and dominant selection marker for neomycin resistance (Neor) was introduced into embryonal carcinoma and embryo-derived cell lines by transfection and infection using pseudotypes with Friend helper virus (Friend murine leukemia virus [F-MuLV]). Cells resistant to G418 (a neomycin analog) were cloned and expanded. Transductants retained an undifferentiated phenotype as judged by morphology, tumorigenicity, and cell-surface antigen analyses. Nucleic acid analysis of infectants revealed both Neor-MPSV and F-MuLV proviruses, although no virus was released. G418-resistant transductants remained nonpermissive for the expression of other proviruses and for subsequent superinfection. Northern analysis showed expression of full-length Neor-MPSV, as well as mos-specific subgenomic RNA. mos sequences were deleted from Neor-MPSV (Neor mos-1), and pseudotypes were used to infect embryonal carcinoma cells. No morphological differences were observed in either mos+ or mos- transductants as compared with parental cell lines. However, mos+ transductants showed an enhanced anchorage-independent growth compared with that of mos- transductants in agar cloning. PCC4 transductants were induced to differentiate with retinoic acid and superinfected with F-MuLV. Infection with viral supernatant in fibroblasts and in mice confirmed the rescue of biologically active Neor-MPSV.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-185623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-202395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-2994046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-3857593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-4000280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-4599372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-4858519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-510422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-5436899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6188535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6243788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6244898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6245244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6246522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6251235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6261151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6265929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6271971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6273072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6287220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6288258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6289129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6292737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6296681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6323996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6328001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6328002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6537818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6541297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6593721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6717601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6727874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6772444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-6955793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-7086331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-720785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-7237558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-7242681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-7269249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-73185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-7414951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023829-872896
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0270-7306
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
6
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
286-93
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:3023829-Animals,
pubmed-meshheading:3023829-Cell Line,
pubmed-meshheading:3023829-Cell Transformation, Neoplastic,
pubmed-meshheading:3023829-Drug Resistance,
pubmed-meshheading:3023829-Embryo, Mammalian,
pubmed-meshheading:3023829-Genes, Viral,
pubmed-meshheading:3023829-Mice,
pubmed-meshheading:3023829-Moloney murine sarcoma virus,
pubmed-meshheading:3023829-Neomycin,
pubmed-meshheading:3023829-Oncogenes,
pubmed-meshheading:3023829-Sarcoma Viruses, Murine,
pubmed-meshheading:3023829-Teratoma,
pubmed-meshheading:3023829-Transcription, Genetic,
pubmed-meshheading:3023829-Transfection
|
|
pubmed:year |
1986
|
|
pubmed:articleTitle |
Viral transfer, transcription, and rescue of a selectable myeloproliferative sarcoma virus in embryonal cell lines: expression of the mos oncogene.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
