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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1987-1-14
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pubmed:databankReference |
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pubmed:abstractText |
The human cytomegalovirus (HCMV)-induced DNA polymerase has been well characterized biochemically and functionally, but its genomic location has not yet been assigned. To identify the coding sequence, cross-hybridization with the herpes simplex virus type 1 (HSV-1) polymerase gene was used, as suggested by the close similarity of the herpes group virus-induced DNA polymerases to the HCMV DNA polymerase. A cosmid and plasmid library of the entire HCMV genome was screened with the BamHI Q fragment of HSV-1 at different stringency conditions. One PstI-HincII restriction fragment of 850 base pairs mapping within the EcoRI M fragment of HCMV cross-hybridized at Tm - 25 degrees C. Sequence analysis revealed one open reading frame spanning the entire sequence. The amino acid sequence showed a highly conserved domain of 133 amino acids shared with the HSV and putative Epstein-Barr virus polymerase sequences. This domain maps within the C-terminal part of the HSV polymerase gene, which has been suggested to contain part of the catalytic center of the enzyme. Transcription analysis revealed one 5.4-kilobase early transcript in the sense orientation with respect to the open reading frame identified. This transcript appears to code for the 140-kilodalton HCMV polymerase protein.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-13768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-168404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-172657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-202961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-20515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-223846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-225553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-230783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-2578573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-2983088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-2985818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-2997930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-2999440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-2999714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-2999787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-3023690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6087149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6091052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6186074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6199514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6208812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6246099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6247818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6264291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6270349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6278309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6286417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6290339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6295880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6302813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6310343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6319573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6319737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6329907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-6621377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-7192534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3023689-881736
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
119-24
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3023689-Amino Acid Sequence,
pubmed-meshheading:3023689-Base Sequence,
pubmed-meshheading:3023689-Cloning, Molecular,
pubmed-meshheading:3023689-Cytomegalovirus,
pubmed-meshheading:3023689-DNA, Recombinant,
pubmed-meshheading:3023689-DNA-Directed DNA Polymerase,
pubmed-meshheading:3023689-Genes,
pubmed-meshheading:3023689-Genes, Viral,
pubmed-meshheading:3023689-Humans,
pubmed-meshheading:3023689-Nucleic Acid Hybridization,
pubmed-meshheading:3023689-Transcription, Genetic
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pubmed:year |
1987
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pubmed:articleTitle |
Genomic localization, sequence analysis, and transcription of the putative human cytomegalovirus DNA polymerase gene.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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