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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1986-12-30
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pubmed:databankReference |
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pubmed:abstractText |
The nucleotide sequence for the human nerve growth factor (NGF) receptor has been determined. The 3.8 kb receptor mRNA encodes a 427 amino acid protein containing a 28 amino acid signal peptide, an extracellular domain containing four 40 amino acid repeats with six cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155 amino acid cytoplasmic domain. The sequence of the extracellular domain of the NGF receptor predicts a highly ordered structure containing a negatively charged region that may serve as the ligand-binding site. This domain is conserved through evolution. Transfection of a full-length cDNA in mouse fibroblasts results in stable expression of NGF receptors that are recognized by monoclonal antibodies to the human NGF receptor and that bind [125I]NGF.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
545-54
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:3022937-Amino Acid Sequence,
pubmed-meshheading:3022937-Animals,
pubmed-meshheading:3022937-Base Sequence,
pubmed-meshheading:3022937-Biological Evolution,
pubmed-meshheading:3022937-Cloning, Molecular,
pubmed-meshheading:3022937-Coturnix,
pubmed-meshheading:3022937-Cysteine,
pubmed-meshheading:3022937-DNA,
pubmed-meshheading:3022937-Gene Expression Regulation,
pubmed-meshheading:3022937-Humans,
pubmed-meshheading:3022937-Membrane Proteins,
pubmed-meshheading:3022937-Nerve Growth Factors,
pubmed-meshheading:3022937-Protein Biosynthesis,
pubmed-meshheading:3022937-Rats,
pubmed-meshheading:3022937-Receptors, Cell Surface,
pubmed-meshheading:3022937-Receptors, Nerve Growth Factor,
pubmed-meshheading:3022937-Sequence Homology, Nucleic Acid,
pubmed-meshheading:3022937-Transfection
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pubmed:year |
1986
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pubmed:articleTitle |
Expression and structure of the human NGF receptor.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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