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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
|
pubmed:dateCreated |
1986-12-11
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pubmed:abstractText |
RNA of a flavivirus-tick-borne encephalitis virus (strain Sofjin) was subjected to reverse transcription and the DNA copy was transformed into double-stranded DNA by action of E. coli DNA-polymerase I (Klenow's fragment). This DNA was annealed with pBR322 plasmid. The recombinant plasmids were cloned in E. coli K802. The nucleotide sequence of the inserts of the clones coding for region of structural proteins C, pre-M, E and nonstructural protein ns1 was determined by the Maxam-Gilbert method. The nucleotide sequence of these regions is translatable into an amino acid sequence of proteins without interruption. The amino acid sequences of proteins and nucleotide sequence of genome of the tick-borne encephalitis virus are extensively homologous to that found for the flaviviruses Yellow Fever and West Nile.
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pubmed:language |
rus
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Restriction Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Structural Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0132-3423
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1189-202
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3022757-Base Sequence,
pubmed-meshheading:3022757-DNA, Viral,
pubmed-meshheading:3022757-DNA Restriction Enzymes,
pubmed-meshheading:3022757-Encephalitis Viruses, Tick-Borne,
pubmed-meshheading:3022757-Genes, Viral,
pubmed-meshheading:3022757-RNA, Viral,
pubmed-meshheading:3022757-Viral Proteins,
pubmed-meshheading:3022757-Viral Structural Proteins,
pubmed-meshheading:3022757-Virion
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pubmed:year |
1986
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pubmed:articleTitle |
[Nucleotide sequence of the genome region of the tick-borne encephalitis virus coding for structural virion proteins].
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pubmed:publicationType |
Journal Article,
English Abstract
|