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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1986-11-20
|
| pubmed:abstractText |
The high-affinity mutant cI gene of lambda cIha (Nag et al. 1984) was cloned in the multicopy plasmid pBR322. In the resulting plasmid, pMD 102, a lacUV5 promoter was inserted giving the lacUV5-cIha fusion plasmid pMD 205. Bacteria carrying pMD 102 and pMD 205 contain 2.5 and 15 times, respectively, the level of repressor in a monolysogen of lambda cIha. Results of the study of certain properties of the bacteria carrying these plasmids suggest that the ha repressor also has a higher affinity for the virulent mutant operators as well as the prm promoter of lambda.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0026-8925
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
204
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
540-2
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3020384-Bacteriophage lambda,
pubmed-meshheading:3020384-Cloning, Molecular,
pubmed-meshheading:3020384-DNA Restriction Enzymes,
pubmed-meshheading:3020384-Escherichia coli,
pubmed-meshheading:3020384-Genes, Viral,
pubmed-meshheading:3020384-Mutation,
pubmed-meshheading:3020384-Plasmids,
pubmed-meshheading:3020384-Repressor Proteins,
pubmed-meshheading:3020384-Transcription Factors
|
| pubmed:year |
1986
|
| pubmed:articleTitle |
Structure and function of the repressor of bacteriophage lambda. III. Molecular cloning of the high-affinity mutant cI gene of lambda and studies of the properties of the clones.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|