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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1986-11-17
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pubmed:abstractText |
Large tumor antigen (T antigen) was extracted from SV40-infected African Green Monkey cells and purified to homogeneity by immunoaffinity chromatography. The purified T antigen preparations unwind DNA duplices of greater than 120 bp in a reaction which is dependent on magnesium ions and ATP hydrolysis. Based on these and other properties of the reaction we classify this newly discovered enzymatic activity as a eukaryotic DNA helicase. The helicase and the known ATPase function of T antigen cosediment with the mono- or dimeric 4-6 S form of T antigen, but not with higher T antigen aggregates. The helicase activity seems to be an intrinsic function of SV40 T antigen. First, several different T antigen-specific monoclonal antibodies interfere with the DNA unwinding activity; monoclonals which are known to reduce the T antigen-specific ATPase most strongly inhibited the helicase reaction. Second, mutant T antigens with impaired ATPase function also showed a reduced DNA unwinding activity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-218212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-218962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-224046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-226311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-226974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-2419584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-2983081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-2985809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-2996217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-2998049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-2998767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-3003386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-3004946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-3162158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-3905387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6093377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6163870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6170639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6175245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6204863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6208378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6253123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6253946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6256739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6260373,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6292844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6300658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6302981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-6306282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3019672-7237559
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1939-44
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3019672-Animals,
pubmed-meshheading:3019672-Antigens, Polyomavirus Transforming,
pubmed-meshheading:3019672-Antigens, Viral, Tumor,
pubmed-meshheading:3019672-Cell Line,
pubmed-meshheading:3019672-Cercopithecus aethiops,
pubmed-meshheading:3019672-DNA Topoisomerases, Type I,
pubmed-meshheading:3019672-Kidney,
pubmed-meshheading:3019672-Kinetics,
pubmed-meshheading:3019672-Oncogene Proteins, Viral,
pubmed-meshheading:3019672-Simian virus 40
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pubmed:year |
1986
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pubmed:articleTitle |
DNA helicase activity of SV40 large tumor antigen.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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