| pubmed-article:3019326 | pubmed:abstractText | The inhibition of pig liver fructose 1,6-bisphosphatase by fructose 2,6-bisphosphate has been investigated over a wide range of substrate concentration by measuring the release of labelled inorganic phosphate from [1-32P]fructose 1,6-bisphosphate. The activity of the enzyme can be inhibited completely by fructose 2,6-bisphosphate. The inhibiting effect is most pronounced at low substrate concentrations. The results have been analyzed in terms of a mathematical model assuming a competitive interaction of fructose 1,6-bisphosphate and fructose 2,6-bisphosphate at the catalytic site as well as a synergistic cooperation of the two hexose bisphosphates at an inhibiting allosteric site of the enzyme. | lld:pubmed |
