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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1986-10-8
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pubmed:abstractText |
In an attempt to place a human beta-globin gene in an open chromatin domain regardless of its site of integration in the mouse genome, we microinjected into fertilized mouse eggs a construct in which the human beta-globin gene and a mouse metallothionein-human growth hormone fusion gene were juxtaposed and oriented in opposite directions. Mice that developed from injected eggs and that grew larger than normal were analyzed for human beta-globin mRNA. The globin genes were not expressed in erythroid tissue but were expressed with the same tissue specificity as metallothionein-human growth hormone. These results suggest that sequences which control metallothionein-human growth hormone gene expression are capable of stimulating the expression of a flanking gene in an orientation-independent and tissue-specific manner. As a control for this experiment, we deleted the metallothionein-human growth hormone transcription unit and noted that the human beta-globin gene then was expressed at high levels with erythroid tissue specificity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-3982505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-4817965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6088069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6095286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6095291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6205764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6254664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6272287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6356363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6358900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6409417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6409418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6409419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6467367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6472463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6614474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6616614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6796959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-6945481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-7388947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018541-922889
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1977-83
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3018541-Animals,
pubmed-meshheading:3018541-DNA Restriction Enzymes,
pubmed-meshheading:3018541-Endonucleases,
pubmed-meshheading:3018541-Genes,
pubmed-meshheading:3018541-Globins,
pubmed-meshheading:3018541-Growth Hormone,
pubmed-meshheading:3018541-Humans,
pubmed-meshheading:3018541-Metallothionein,
pubmed-meshheading:3018541-Mice,
pubmed-meshheading:3018541-Microinjections,
pubmed-meshheading:3018541-Nucleic Acid Hybridization,
pubmed-meshheading:3018541-Plasmids,
pubmed-meshheading:3018541-RNA, Messenger,
pubmed-meshheading:3018541-Single-Strand Specific DNA and RNA Endonucleases,
pubmed-meshheading:3018541-Tissue Distribution,
pubmed-meshheading:3018541-Transcription, Genetic
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pubmed:year |
1985
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pubmed:articleTitle |
Expression of human beta-globin genes in transgenic mice: effects of a flanking metallothionein-human growth hormone fusion gene.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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