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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1986-10-3
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pubmed:abstractText |
The McDonough strain of feline sarcoma virus contains an oncogene called v-fms whose ultimate protein product (gp140v-fms) resembles a cell surface growth factor receptor. To identify and characterize the protein product of the proto-oncogene c-fms, antisera were prepared to the viral fms sequences and used to detect specific cross-reacting sequences in human choriocarcinoma cells (BeWo) known to express c-fms mRNA. Both tumor-bearing rat sera and a rabbit antiserum prepared to a segment of v-fms expressed in Escherichia coli detected a 140-kilodalton (kDa) glycoprotein in the BeWo cells. Tryptic fingerprint analysis of [35S]methionine-labeled proteins indicated that the viral fms proteins and the 140-kDa BeWo cell protein were highly related. This 140-kDa glycoprotein contained an associated tyrosine kinase activity in vitro and was labeled principally on serine after 32Pi metabolic labeling. These results suggest that the 140-kDa protein in BeWo cells is the protein product of the human c-fms proto-oncogene. This conclusion is supported by the finding that a similar protein is detectable only in other human cells that express c-fms mRNA. These other human cells include adherent monocytes and the cell line ML-1, which can be induced to differentiate along the monocyte-macrophage pathway. This is in agreement with current thought that the c-fms proto-oncogene product functions as the CSF-1 receptor specific to this pathway.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-1174689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-214578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-225544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-2408759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-2580348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-276884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-288066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-3018522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-3986905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-4179068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-4299001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-4372784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-4997847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-5057599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6205276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6272395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6275118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6281462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6292527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6297801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6302985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6320011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6328316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6351725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6356358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6361277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6381756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6411728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6431300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6538699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6582485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6632085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6683787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6958361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-6978185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-7068848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018521-978727
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3458-66
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:3018521-Antigens,
pubmed-meshheading:3018521-Cross Reactions,
pubmed-meshheading:3018521-Gene Expression Regulation,
pubmed-meshheading:3018521-Humans,
pubmed-meshheading:3018521-Macrophages,
pubmed-meshheading:3018521-Monocytes,
pubmed-meshheading:3018521-Oncogene Proteins, Viral,
pubmed-meshheading:3018521-Protein-Tyrosine Kinases,
pubmed-meshheading:3018521-Proto-Oncogene Proteins,
pubmed-meshheading:3018521-Proto-Oncogenes,
pubmed-meshheading:3018521-RNA, Messenger,
pubmed-meshheading:3018521-Sarcoma Viruses, Feline
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pubmed:year |
1985
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pubmed:articleTitle |
Characterization of the human c-fms gene product and its expression in cells of the monocyte-macrophage lineage.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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