|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0001483,
umls-concept:C0007634,
umls-concept:C0011155,
umls-concept:C0014239,
umls-concept:C0018270,
umls-concept:C0028584,
umls-concept:C0041361,
umls-concept:C0162867,
umls-concept:C0596235,
umls-concept:C1510411,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636
|
|
pubmed:issue |
11
|
|
pubmed:dateCreated |
1986-9-29
|
|
pubmed:abstractText |
Rat embryo cell lines containing the adenovirus 2 E1a region together with normal or mutant forms of the N-terminal half of the E1b region (HindIII G fragment) were generated by using a dominant selection marker, neo. Biochemically transformed cells containing a nonmutated HindIII G fragment proliferated more rapidly in Ca2+-deficient media, whereas cells containing a specific deletion within the E1b-encoded, 175-amino-acid (175R) (19-kilodalton) T-antigen gene and nontransformed cells grew at a slower rate. Furthermore, transformed cells that did not express the 175R T antigen and untransformed cells could not replicate their DNA efficiently in low-Ca2+ medium. Our results suggest that Ca2+ ions may provide an important stimulus for cell proliferation in adenovirus-transformed cells through a mechanism that involves the functions of the 175R T antigen.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-4473721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-5723670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-5890603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-608590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6095092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6146314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6271971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6274343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6286831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6306914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6317888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6328320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6333514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6396510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6492253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6492262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6605482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6834478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6854738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6871992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-6954499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-7037065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-7097863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3018514-7423858
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0270-7306
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
5
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3297-300
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:3018514-Adenoviruses, Human,
pubmed-meshheading:3018514-Animals,
pubmed-meshheading:3018514-Antigens, Neoplasm,
pubmed-meshheading:3018514-Calcium,
pubmed-meshheading:3018514-Cell Division,
pubmed-meshheading:3018514-Cell Line,
pubmed-meshheading:3018514-Cell Transformation, Viral,
pubmed-meshheading:3018514-Culture Media,
pubmed-meshheading:3018514-DNA Replication,
pubmed-meshheading:3018514-DNA Restriction Enzymes,
pubmed-meshheading:3018514-Embryo, Mammalian,
pubmed-meshheading:3018514-Endoplasmic Reticulum,
pubmed-meshheading:3018514-Kinetics,
pubmed-meshheading:3018514-Mutation,
pubmed-meshheading:3018514-Nuclear Envelope,
pubmed-meshheading:3018514-Rats
|
|
pubmed:year |
1985
|
|
pubmed:articleTitle |
An adenovirus 2-coded tumor antigen located on the endoplasmic reticulum and nuclear envelope is required for growth of transformed cells in Ca2+-deficient media.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
