| pubmed-article:3017972 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3017972 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:3017972 | lifeskim:mentions | umls-concept:C0220806 | lld:lifeskim |
| pubmed-article:3017972 | lifeskim:mentions | umls-concept:C0021757 | lld:lifeskim |
| pubmed-article:3017972 | lifeskim:mentions | umls-concept:C0079459 | lld:lifeskim |
| pubmed-article:3017972 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:3017972 | lifeskim:mentions | umls-concept:C0018284 | lld:lifeskim |
| pubmed-article:3017972 | pubmed:issue | 26 | lld:pubmed |
| pubmed-article:3017972 | pubmed:dateCreated | 1986-10-17 | lld:pubmed |
| pubmed-article:3017972 | pubmed:abstractText | Granulocyte colony-stimulating factor (G-CSF) and multipotential colony-stimulating factor (multi-CSF or interleukin 3) are two members of a family of hemopoietic growth and differentiation factors. Using biologically active radioiodinated derivatives and chemical cross-linking (predominantly with the homobifunctional reagent disuccinimidyl suberate) followed by gel electrophoresis and autoradiography, receptors for these two factors have been identified. The G-CSF receptor was identified as a single subunit protein of Mr approximately 150,000 while two molecular species able to specifically cross-link to 125I-multi-CSF were identified of Mr approximately 75,000 and 60,000. For both CSFs specificity of formation of cross-linked species was demonstrated by showing that the homologous unlabeled CSF (but not other CSFs) competed for formation of the complexes with the appropriate dose-response relation, by showing that saturation occurred over the appropriate range of 125I-CSF concentration and by showing that the cellular specificity of CSF binding paralleled that for cross-linked complex formation. The formation of cross-linked complexes was dependent on the concentration and type of chemical cross-linker, especially for cross-linking of 125I-multi-CSF. Based on a number of criteria it is suggested that the two species cross-linked to 125I-multi-CSF do not represent receptors of different affinity but, rather, two noncovalently associated subunits of a receptor complex. | lld:pubmed |
| pubmed-article:3017972 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3017972 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3017972 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3017972 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3017972 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3017972 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:3017972 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3017972 | pubmed:author | pubmed-author:NicolaN ANA | lld:pubmed |
| pubmed-article:3017972 | pubmed:author | pubmed-author:PetersonLL | lld:pubmed |
| pubmed-article:3017972 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3017972 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:3017972 | pubmed:volume | 261 | lld:pubmed |
| pubmed-article:3017972 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3017972 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3017972 | pubmed:pagination | 12384-9 | lld:pubmed |
| pubmed-article:3017972 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:3017972 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3017972 | pubmed:articleTitle | Identification of distinct receptors for two hemopoietic growth factors (granulocyte colony-stimulating factor and multipotential colony-stimulating factor) by chemical cross-linking. | lld:pubmed |
| pubmed-article:3017972 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3017972 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3017972 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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