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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1986-10-15
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pubmed:abstractText |
Monoclonal antibodies (MAbs) of two different specificities were produced by immunizing mice with the semipurified M antigen of histoplasmin. One type, from clone CB4, was an immunoglobulin M that precipitated a polysaccharide present in histoplasmin and also formed immunoprecipitates with a cross-reactive polysaccharide present in extracts of Blastomyces dermatitidis and Coccidioides immitis. The second type of MAb, from clone EC2, was an immunoglobulin G that reacted in the enzyme-linked immunoelectrotransfer blot (EITB) assay with a doublet of proteins with an apparent molecular size of 70 to 75 kilodaltons. This molecule is proposed as the authentic M protein antigen that is recognized by M antibodies in sera from mice and rabbits immunized with Histoplasma capsulatum and from persons with histoplasmosis. The M factor also occurs in an abundant disulfide-bridged dimer which has a molecular size of 150 kilodaltons and is nonimmunoreactive under the conditions of sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
540-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3017863-Animals,
pubmed-meshheading:3017863-Antibodies, Monoclonal,
pubmed-meshheading:3017863-Antigens, Fungal,
pubmed-meshheading:3017863-Carbohydrates,
pubmed-meshheading:3017863-Dithiothreitol,
pubmed-meshheading:3017863-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3017863-Female,
pubmed-meshheading:3017863-Fungal Proteins,
pubmed-meshheading:3017863-Histoplasmin,
pubmed-meshheading:3017863-Humans,
pubmed-meshheading:3017863-Immunoenzyme Techniques,
pubmed-meshheading:3017863-Isoelectric Focusing,
pubmed-meshheading:3017863-Mice,
pubmed-meshheading:3017863-Mice, Inbred BALB C,
pubmed-meshheading:3017863-Periodic Acid,
pubmed-meshheading:3017863-Precipitin Tests
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pubmed:year |
1986
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pubmed:articleTitle |
Monoclonal antibodies against the M-protein and carbohydrate antigens of histoplasmin characterized by the enzyme-linked immunoelectrotransfer blot method.
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pubmed:publicationType |
Journal Article
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