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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
1986-9-17
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pubmed:abstractText |
The MES-1 element was previously isolated from restricted total mouse cellular DNA by "expression selection"--the ability to reactivate expression of a test gene devoid of its 5' enhancer sequences. Mes-1 has been tested in long-term transformation and short-term CAT expression assays. In both assays MES-1 is active independent of orientation and at a distance when placed 5' to the test gene. The element is active with heterologous promoters and functions efficiently in both rat and mouse cells. MES-1 activates expression by increasing transcription from the test gene's own start (cap) site. Thus the expression selection technique can be used for the isolation of DNA sequences with enhancer-like properties from total cellular DNA.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-2414012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-2419129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-2985280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-2987925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-2990916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-2996884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-3001696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-3904002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-390497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-3986910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-4041012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6091340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6233005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6273820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6275354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6277502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6292433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6300895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6304653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6308429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6323032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6325170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6690710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3016657-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5615-27
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3016657-Acetyltransferases,
pubmed-meshheading:3016657-Ampicillin,
pubmed-meshheading:3016657-Animals,
pubmed-meshheading:3016657-Base Sequence,
pubmed-meshheading:3016657-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:3016657-DNA Restriction Enzymes,
pubmed-meshheading:3016657-Enhancer Elements, Genetic,
pubmed-meshheading:3016657-Escherichia coli,
pubmed-meshheading:3016657-Genes, Regulator,
pubmed-meshheading:3016657-Genetic Vectors,
pubmed-meshheading:3016657-Mice,
pubmed-meshheading:3016657-Penicillin Resistance,
pubmed-meshheading:3016657-Plasmids,
pubmed-meshheading:3016657-Transcription, Genetic
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pubmed:year |
1986
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pubmed:articleTitle |
Gene activation properties of a mouse DNA sequence isolated by expression selection.
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pubmed:publicationType |
Journal Article
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