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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1986-9-16
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pubmed:abstractText |
To define the extent of intervening sequences required for efficient splicing of the CYH2 gene in Saccharomyces cerevisiae, we have constructed a series of intron mutations. Artificial intron extensions of more than 300 bp of the natural intron lead to an inhibition of splicing whereas intron deletions lead to a drastic improvement of the splicing efficiency. It is shown that deletion of a 32 bp sequence element within the intron is responsible for this drastic improvement.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
May
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pubmed:issn |
0026-8925
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
203
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
300-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3016482-Base Sequence,
pubmed-meshheading:3016482-DNA Restriction Enzymes,
pubmed-meshheading:3016482-DNA Transposable Elements,
pubmed-meshheading:3016482-Genes, Fungal,
pubmed-meshheading:3016482-Genetic Vectors,
pubmed-meshheading:3016482-Mutation,
pubmed-meshheading:3016482-Nucleic Acid Hybridization,
pubmed-meshheading:3016482-Plasmids,
pubmed-meshheading:3016482-RNA Splicing,
pubmed-meshheading:3016482-Saccharomyces cerevisiae
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pubmed:year |
1986
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pubmed:articleTitle |
Intron mutations that affect the splicing efficiency of the CYH2 gene of Saccharomyces cerevisiae.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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