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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1986-9-17
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pubmed:abstractText |
Activated glucocorticoid receptor protein (GCR) was partially purified from porcine liver cytosol by sequential chromatography on phosphocellulose and DNA-cellulose using a modification of a protocol developed for purification of rat GCR. This partially purified preparation, when separated by SDS-polyacrylamide gel electrophoresis and immunoblotted, indicated that a Mr = 94,000 protein band cross-reacts with a monoclonal antibody against rat GCR. A nitrocellulose filter binding assay showed that both the partially purified porcine and rat GCRs interact specifically with a cloned synthetic 24 base pair deoxyoligonucleotide containing the GCR binding sequence in the first intron of the human growth hormone (hGH) gene. This specific protein-DNA interaction is blocked by a single base pair change in the binding site. All three putative domains of the GCR molecule: the steroid binding, immunoreactive, and DNA binding have been conserved between two divergent species.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-4731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1097-103
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3016408-Animals,
pubmed-meshheading:3016408-Antibodies, Monoclonal,
pubmed-meshheading:3016408-Base Sequence,
pubmed-meshheading:3016408-Chromatography, Affinity,
pubmed-meshheading:3016408-Collodion,
pubmed-meshheading:3016408-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3016408-Immunologic Techniques,
pubmed-meshheading:3016408-Male,
pubmed-meshheading:3016408-Mutation,
pubmed-meshheading:3016408-Rats,
pubmed-meshheading:3016408-Receptors, Cell Surface,
pubmed-meshheading:3016408-Receptors, Glucocorticoid,
pubmed-meshheading:3016408-Receptors, Somatotropin,
pubmed-meshheading:3016408-Species Specificity,
pubmed-meshheading:3016408-Swine
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pubmed:year |
1986
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pubmed:articleTitle |
Conservation of the DNA binding domain and other properties between porcine and rat glucocorticoid receptors.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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