Linked Life Data

3014728

Source:http://linkedlifedata.com/resource/pubmed/id/3014728

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1986-8-14
pubmed:abstractText
The 24-kDa cap binding protein (CBP) from uninfected, mock-infected and poliovirus-infected HeLa cell extracts was isolated by m7GTP affinity chromatography and examined by isoelectric focusing followed by SDS-PAGE. Two major species (pI 6.7 and 7.1) and two minor species (pI 6.5 and 6.8) were found in all cases. Preparations from postribosomal supernate (S200) and the supernate from 0.5 M KCl washed ribosomes (RSW) also demonstrated these same four species. We conclude that there are no detectable differences between ribosome-associated and soluble 24-kDa CBP and that the 24-kDa CBP is not detectably altered by poliovirus infection. We also report the presence of a previously undescribed 16-kDa polypeptide(s) doublet that copurifies with the 24-kDa CBP from uninfected, mock-infected and poliovirus-infected HeLa cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
152
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
497-501
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Two-dimensional gel analyses of the 24-kDa cap binding protein from poliovirus-infected and uninfected HeLa cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.