Linked Life Data

3014315

Source:http://linkedlifedata.com/resource/pubmed/id/3014315

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-7-25
pubmed:abstractText
The effect of DNA superhelicity on the transcription of T4 DNA fragments containing early genes uvs W, Y and late genes 25-29 was studied. RNA polymerase transcribes both early and late phage genes within the supercoiled recombinant plasmid. Late genes relative transcription increases essentially when T4-modified RNA polymerase is used. DNA relaxation causes a sharp decrease of modified RNA polymerase activity, especially on the late genes. The same effect is obtained in intact cells with recombinant plasmid, which superhelicity is lowered by temperature-sensitive mutation in DNA gyrase. The data obtained prove that DNA superhelicity is required for the T4 late transcription by phage-modified RNA polymerase. It is suggested that the dependence of late transcription on the phage DNA replication in infected cells is connected with phage DNA superspiralization throughout its replication.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0026-8984
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
745-50
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
[Effect of superhelical DNA on the transcription of cloned genes of the T4 phage].
pubmed:publicationType
Journal Article, English Abstract