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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1977-7-18
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pubmed:abstractText |
Supernates of human T cells stimulated with TT antigen contain a factor that induces mitogenesis and immunoglobulin synthesis in autologous as well as allogeneic B cells. A fraction of the IgG produced has specificity against TT. The T-cell-derived LMF-TT eluted after albumin on Sephadex G 200 and did not contain immunoglobulin heavy chain determinants. LMF-TT was active on B cells from TT immune as well as TT- nonimmune individuals but in the latter instance the IgG secreted had no specificity against TT. B cells incubated with LMF-TT in the presence of a second antigen (DT) made IgG with specifity to that antigen provided the B-cell donor was immune to that second antigen. LMF-TT-containing supernates were depleted of TT antigen by Sephadex G 200 chromatography followed by passage over anti-TT immunosorbent columns. The antigen-free supernates were able to induce mitogenesis and IgG synthesis in B cells but the IgG produced failed to exhibit specificity against TT unless the TT antigen was readded to the B-cell cultures. The optimal concentration of LMF-TT (50 percent) inducing B-cell mitogenesis was different from the optimal concentration (20 percent) causing IgG synthesis by B cells. At low LMF concentrations (less than or equal 10 percent) addition of a second antigen to which the cell donor was immune caused an increase in the degree of B-cell mitogenesis. Submitogenic concentrations of LMF-TT (less than or equal to 5 percent) were still capable of inducingimmunoglobulin synthesis in B cells At these low concentrations of LMF-TT the proportion of anti-TT IgG over total IgG increased sharply. B cells from TT immune donors were separated on TT immunosorbent columns. Cells that bound to the column were more sensitive to the mitogenic and IgG synthetic effects of LMF-TT than unfractionated B cells. Thus, LMF is a nonspecific human T-cell helper factor which behaves like a polyclonal B-cell activator. However, in the presence of specific antigen (TT) the antigen-specific B cell is preferentially triggered by LMF. The experimental design of the present study does not rule out the additional presence of an antigen-specific helper factor in the supernates of TT-stimulated human T cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-1082098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-1089726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-1092612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-1096383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-1100764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-14299034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-4132599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-4169033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-4200776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-4273650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-4503682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-4545893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-4578158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-4594643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-4605203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-4606790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-4610078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-5066511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-5531224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-5540515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-58031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-58834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/301172-792335
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
145
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1436-48
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pubmed:dateRevised |
2010-6-22
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pubmed:meshHeading |
pubmed-meshheading:301172-Antibody Formation,
pubmed-meshheading:301172-Antibody-Producing Cells,
pubmed-meshheading:301172-Antigens,
pubmed-meshheading:301172-B-Lymphocytes,
pubmed-meshheading:301172-DNA,
pubmed-meshheading:301172-Dose-Response Relationship, Drug,
pubmed-meshheading:301172-Humans,
pubmed-meshheading:301172-Immunity,
pubmed-meshheading:301172-Immunoglobulin G,
pubmed-meshheading:301172-Immunosorbents,
pubmed-meshheading:301172-Mitosis,
pubmed-meshheading:301172-T-Lymphocytes,
pubmed-meshheading:301172-Tetanus Toxoid
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pubmed:year |
1977
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pubmed:articleTitle |
The specificity of T-cell helper factor in man.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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