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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0018270,
umls-concept:C0029341,
umls-concept:C0033684,
umls-concept:C0185117,
umls-concept:C0205171,
umls-concept:C0521026,
umls-concept:C0596988,
umls-concept:C1335439,
umls-concept:C2911684
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pubmed:issue |
8
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pubmed:dateCreated |
1986-5-23
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pubmed:abstractText |
Transformed cell lines derived from murine C127 cells were constructed that express the influenza virus RNA-dependent RNA polymerase proteins (PA, PB1, and PB2). Cell lines that express only one or all three of the proteins were tested for their ability to complement temperature-sensitive viral mutants incubated at the nonpermissive temperature. Two cell lines were isolated that express all three polymerase genes and complement the growth of PB2 temperature-sensitive mutants at the nonpermissive temperature. One of these lines also complemented PA temperature-sensitive mutants. The viral titers obtained in these two cell lines were 12-fold to 1000-fold higher than the viral titers obtained upon growth of the corresponding temperature-sensitive mutant in C127 cells at the nonpermissive temperature.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-1165595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-1168970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-13130792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-225559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-293742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-2989815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-2990897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-4374446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-4673486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-4831907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-6091052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-6260373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-6267479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-6300840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-6319020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-6325880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-6327055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-6616622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-6823015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-6935664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-6950380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-7133998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-841862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-850306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3010315-985644
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2709-13
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3010315-Animals,
pubmed-meshheading:3010315-Bovine papillomavirus 1,
pubmed-meshheading:3010315-Cell Line,
pubmed-meshheading:3010315-Cloning, Molecular,
pubmed-meshheading:3010315-Genes, Viral,
pubmed-meshheading:3010315-Genetic Complementation Test,
pubmed-meshheading:3010315-Genetic Vectors,
pubmed-meshheading:3010315-Mice,
pubmed-meshheading:3010315-Mutation,
pubmed-meshheading:3010315-Orthomyxoviridae,
pubmed-meshheading:3010315-RNA, Viral,
pubmed-meshheading:3010315-RNA Nucleotidyltransferases,
pubmed-meshheading:3010315-RNA Replicase,
pubmed-meshheading:3010315-Virus Replication
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pubmed:year |
1986
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pubmed:articleTitle |
Expression of the three influenza virus polymerase proteins in a single cell allows growth complementation of viral mutants.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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